Application

Anti-Goat IgG (whole molecule)−FITC antibody has been used:in double labeling for laminin and α-fetoprotein (AFP) at a dilution of 1:400in immunohistochemistry at a dilution of 1:50in immunocytochemistryin immunochemical jagged-1 analysesin immunofluorescence at a dilution of 1:200

Anti-goat IgG antibody can be used in antisera localization for neurofilament antigens obtained in rat peripheral nerve and can be done in tissues of rat and human peripheral and central nervous system by the aid of indirect immunofluorescence.

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.

Immunoglobulin G (IgG) stimulates the classical pathway of the complement system. MaternalIgGis transferred to thefetus through the placenta. This is vital fortheimmunedefenseof the neonate against infections. IgGregulates antibody-dependent cell-mediated cytotoxicity (ADCC) and might be associated with allergy. It nullifies virus particles and toxins. Digestion of IgG by papain produces fragment antigen-binding (Fab). Pepsin digestion of IgG generates crystallizable(fc) fragment and a single dimeric F(ab)2. IgG levelsinsevere acute respiratory syndrome coronavirus (SARS-CoV)-2 infected patients may be used as a possible tool to assess the severity and prognosis ofcoronavirus disease 2019 (COVID-19).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Immunoglobulin G (IgG) is found in blood and has four polypeptide chains. It contains two identical 25 kDa κ or λ light (L)chains and two identical 50 kDa γ heavy (H) chains. The light and heavy chains areinterconnected by disulfide bonds. It is the most important type ofglycoproteinantibody present in human serum, secreted by the Bcells. IgG exists infourisotypes:IgG1, IgG2, IgG3, and IgG4. IgGhas the longest serum half-lifecompared to otherimmunoglobulins.

IgG is a major class of immunoglobulin. Goat IgG has two subclasses- IgG1 and IgG2.

IgG antibodies are smallest and are found in all body fluids and capable of fighting bacterial and viral infection. It is the only type of antibody able to cross the placenta and immunise the foetus. The anti-goat IgG (whole molecule)-FITC antibody is useful in indirect immunofluorescence studies of immunised goat sera infected Trichostrongylus colubriformis (nematode). Antigens can be measured by quantitative immunofluorescence using image analysis, in tissue sections stained with FITC labelled rabbit anti goat IgG. Anti-goat IgG antibody reacts specifically with all goat immunoglobulins.

Immunogen

IgG purified from Goat

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.